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Searching archives in the UK to track previous owners.

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Paint media: Analysis of paint samples to try to determine what binding medium has been used. 

Roman emperor Domitian, Leonardo da Vinci, Titian, Tizian, Tiziano Vecellio, Roman emperors, Eleven Caesars, Undici Cesari, Domitianus, Isabella d’Este, Federico II Gonzaga, Mantova, Mantua, Italy, Francis I, Amboise France, Charles I England.